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Hi all,
We have some mis-registration problems also, using unidirectional
multitracking mode. The pinhole was adjusted and the problem presists.
There was a question of fiber optic alignment, but the 405 laser, which uses
a separate fiber optic cable, is not the only channel that has a
registration problem. Alignment of the lasers themselves could be a source
of trouble. Zeiss is currently examining images and mulling over what the
next step in troubleshooting is. Wish us all luck.
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
----- Original Message -----
From: "zhan Tom" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, March 23, 2006 11:10 PM
Subject: Re: all kinds of problems with zeiss lsm510 meta
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> thank you all.I find meta have another problem.When I use multitrack,the
> image from different tracks can not merge well.there is about 0.5um in
> XY dimension,and 2um in Z direction.I test with 1um fluorescent beads.
>
>
>>From: MICHAEL J DELANNOY <[log in to unmask]>
>>Reply-To: Confocal Microscopy List <[log in to unmask]>
>>To: [log in to unmask]
>>Subject: Re: all kinds of problems with zeiss lsm510 meta
>>Date: Thu, 23 Mar 2006 10:46:15 -0500
>>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>TZ
>>We just got a new Meta and yes we have the same
>>problem. The cure was to leave the power switches to
>>the lasers and the cpu on (unfortunately the mercury also)
>>and shut off only the lasers in the software for the day.
>>At night we shut down everything. I too am dissapointed
>>with their remedy.
>>
>>Mike D
>>
>>----- Original Message -----
>>From: zhan Tom <[log in to unmask]>
>>Date: Thursday, March 23, 2006 2:29 am
>>Subject: all kinds of problems with zeiss lsm510 meta
>>
>> > Search the CONFOCAL archive at
>> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> >
>> > hello,
>> > recently our lsm510 meta meet all kinds of problems,it always
>> > show
>> > error log during using."objective com1","videoswitch","objective
>> > time out
>> > ","HGHGSA?",and so on.sometimes it can not boot. I have called
>> > their
>> > engineer,they tell me it is normal,just software bug.Really? I have
>> > to
>> > complain about the bad service.The machine almost doesn't
>> > work,zeiss just
>> > say it is normal ,it is only software bug. Too bad service.
>> >
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