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Hi Griffin,

I got it. Thank you very much. That save me a lot time. Thank you again 
for your help.


Jing


On 1 Mar 06, at 4:34 PM, Perry, Griffin M wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Jing,
>
> The best way that we have found (using Version 3.0 Build 461) is to 
> save
> it as a .tiff.  Under .tiff, Save Options, Save as RGB Format.  Some
> programs don't like the RAW Format.  I haven't saved and tested RAW
> format with Adobe Photoshop, so it might be worth a try.  From here, 
> you
> label which colour for which channel (ours right now (488)Red 2,
> (568)Green 1, (633)Blue 3, but you can change it to whatever you like).
> Finish saving the .tiff.
>
> In Adobe Photoshop, there should be three different colours in the
> picture.  If you want to isolate the three different channels (colours)
> from one another, there are many ways in Adobe Photoshop CS (8.0).
>
> The way I isolate different channels is to make a copy of the original
> image. With the copy selected, go to Image->Adjustment->Levels and pick
> which channel you DON'T want (if you just want green select red).  On
> the output, change them both to 0.  This will take out the red.  Next,
> select blue and set output to 0.  In the copied image, you will only 
> see
> the green channel.
>
> Hope this helps,
>
> G
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] 
> On
> Behalf Of JingHe
> Sent: Wednesday, March 01, 2006 2:29 PM
> To: [log in to unmask]
> Subject: Nikon EZ-C1
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello,
> I'm using Nikon EZ-C1 2.20 to get three different channel pictures. Is
> there any way I could also open them in adobe photoshop in three
> different color pictures? Thank you.
>
> Jing
>