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Dear Microscopists:
I hope you can help me with these questions with your experience.

The small ones:
1) Eukitt mounting - is it good for fluorescence work or does it produce 
background?
2) Regarding fixation of cells on the slides (after cytospin). The protocol 
i've been using is 1% PFA for 30 min RT. We need a rather light fixation 
that keeps the cell morphology as much as possible (to see Live/apo/nec 
changes), and we don't need long term storage, a some months only. I saw in 
Current protocols they recommend 2% for 10 min. Any suggestions of how to 
reach the best possible protocol would be greatly appreciated.

The hard one:
The fluorescent microscope I have been mostly working on (Axiovert) has a 
problem that is driving me nuts: The focus on the eyepieces is different 
than the one in the camera. Therefore, when i am on sharp focus in view, the 
preview on the monitor is out of focus, and i have to focus while looking at 
the screen. This done at 100 msec intervals is somehow bearable, but you can 
guess that my fluorescent pictures are suffering a lot. Besides asking the 
univ. to call a serviceman, is there any quick and dirty way to take care of 
this?

Many thanks!

Uriel.

Uriel Trahtemberg
MD/PhD student
The Laboratory for Cellular and Molecular Immunology
The Hebrew University - Hadassah Medical Organization
Jerusalem - ISRAEL

"God does not care about our mathematical difficulties. He integrates 
empirically."
 Albert Einstein