CONFOCALMICROSCOPY Archives

March 2006

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: Warner RC-30 vs. Bioptechs FCS2
From:
David Knecht-charter <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 5 Mar 2006 10:11:09 -0500
Content-Type:
text/plain
Parts/Attachments:
text/plain (33 lines) 
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We have used both DMEM and RPMI without bicarbonate plus 25mM HEPES.  
Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

On Mar 4, 2006, at 10:31 PM, Randy Learish wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> David
> I am using phenol-red free DMEM/F12 with both HEPES (15mM) and  
> bicarbonate
> (14.3 mM).   Why should I expect better pH stability in the Solent  
> system
> without bicarbonate?
>
> What HEPES concentration do you use?
>
> Thanks again
>
> Randy

ATOM RSS1 RSS2