CONFOCALMICROSCOPY Archives

March 2006

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From:
Julian Smith III <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 1 Mar 2006 12:40:56 -0500
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I am told by our Zeiss rep that Zeiss' new software will take care of 
this problem.  He wasn't sure about the delivery date on the 
software, however.  Would Zeiss rep like to chime in here?
Julian

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Maybe I can add a short word of explanation, since I worked on 
>"in-focus stacking" myself (i.e. merging images from different focal 
>planes in order to get an overall sharp image of the object). Doing 
>this with a stereomicroscope (such as the MZ16FA) is generally not a 
>good idea, because of parallax problems. Indeed, the optical paths 
>to the oculars and the camera are not parallel to the optical axis 
>of the system, which introduces shifts between different focal 
>planes. One reason for which I suggested the MacroFluo was that it 
>uses vertical optical paths and thus it does not have this 
>limitation.
>
>Cedric Vonesch
>PhD student
>Biomedical Imaging Group
>EPFL-STI-IOA-LIB
>BM 4.138 (Batiment BM)
>Station 17
>CH-1015 Lausanne
>Switzerland
>
>Phone: +41 (0)21 693 51 36
>Fax: +41 (0)21 693 37 01
>
>
>
>Diana Kittleson wrote:
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>  Hello,
>>I am evaluating macroscopes capable of in-focus stacking for bright
>>field and fluorescence applications.  I have looked at the Olympus MVX10
>>and the Leica MZ16FA.  The demo Olympus instrument did not have a
>>z-motor which makes for a difficult comparison.  I would sincerely
>>appreciate any comments on your satisfaction or recommendations for such
>>a purchase.
>>Thank you kindly,
>>Diana
>>
>>Diana Kittleson
>>Strategic Technology
>>General Mills Riverside Technology
>>330 University Avenue Southeast
>>Minneapolis, Minnesota 55414
>>763-764-2330
>>763-764-2331 fax
>>
>>


-- 
Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC  29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)

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