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Hello listers,
We have a Zeiss LSM 510 system without a UV laser or two-photon laser. I
would like to learn your suggestions on which DNA dye excited by visible
laser (Argon or HeNe) is the best substitute of DAPI.
Thanks.
-------------------------
Zifu Wang, Ph.D.
Optical Biology Core Facility
Developmental Biology Center
2114 McGaugh Hall
Univerity of California
Irvine, CA 92697-2275
Tel: (949) 824-3856
FAx:(949) 824-3571
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Guy Cox
Sent: Monday, May 01, 2006 10:45 PM
To: [log in to unmask]
Subject: Re: propidium iodide and death
Search the CONFOCAL archive at
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I'm not sure I can follow this argument. I certainly
cannot divide (nor indeed reproduce any other way since
I've had a vasectomy) and at 61 I'd say I'm pretty
much fully differentiated. But I've heard nobody
suggest that I'm not alive.
Guy
Joel Sheffield wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> You raise an interesting philosophical question. Is a mature nerve
> cell, or other fully differentiated cell, living or dead? I suppose
> that one could escape the paradox by stating that the "appropriate
> conditions" to support division of these cell types can not be found,
> but this seems more like an evasion than an answer. Perhaps the term
> itself is too vague, and we should look at the use of the term,
> "division competent" as a possibility. We should certainly accept
> the possibility that "living" might also mean "functional",
> "metabolically active", etc.
>
> Joel
>
>
>
> Date sent: Fri, 28 Apr 2006 14:29:25 -0400
> Send reply to: Confocal Microscopy List
<[log in to unmask]>
> From: "Robert J. Palmer Jr." <[log in to unmask]>
> Subject: propidium iodide and death
> To: [log in to unmask]
>
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>PI has a tradition in eukaryotic biology as an indicator of cell death
>>through membrane disruption. I am interested in whether any list
>>watchers can tell me of cases in which strong PI staining is not an
>>indicator of cell death. If many examples of PI staining of growing
>>(or growth-capable) cells exist, then of what is PI staining
>>indicative other than a (transient) membrane permeability difference
>>compared to untreated controls? FYI, my definition of cell death is
>>the inability of cells to divide when provided with appropriate
>>conditions. I am less interested in other measurements of viability -
>>"dividing or dead" is my motto... Thanks for your opinions..... Rob
>>Palmer -- Robert J. Palmer Jr., Ph.D. Natl Inst Dental Craniofacial
>>Res - Natl Insts Health Oral Infection and Immunity Branch Bldg 30,
>>Room 310 30 Convent Drive Bethesda MD 20892 ph 301-594-0025 fax
>>301-402-0396
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [log in to unmask]
--
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
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