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Dear users of confocal microscopy,


some of you are also in charge of facilities and teach people how to use 
a confocal. So I wonder how you do it - more precise, what you are using 
as samples.

What do you suggest? For learning the technique, it should be 
photostable, multiple stained and rather thick to demonstrate Z-scan 
functions.

Since quite some time we use 20 µm kidney sections from Molecular 
Probes, stained with 2x Alexa and DAPI. They are quite photostable - 
but, on the other side, the DAPI staining is not the best.

So I'm looking for some alternatives...

Are there other, better samples on the market? What are your experiences?

Or do you prepare your own samples? If so, I would be interested in some 
protocols.


cheers,
Michael

MTZ Imaging / MPI-CBG LMF
Dresden, Germany