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I don't know what hardware is available to you, but
second harmonic imaging shows starch up very well,
with no staining:
Guy Cox, Nuno Moreno & José Feijó, 2005. Second harmonic
imaging of plant polysaccharides. Journal of Biomedical
Optics 10, 024013_1-6
Trouble is you need acces to a multiphoton microscope
with an expensive titanium sapphire laser but I guess
there might well be one in the University of Milan.
Guy Cox
> Dear all
> I'm a food technologist and my field of research is Cereal Science
> and Technology. In the recent years I've approached to Scanning
> Electron Microscopy and I've found it very fascinating and really
> useful to my research. I'd like to go in depth with the knowledge of
> my materials by using the Confocal Laser Scanning Microscopy: I had
> just a short "meeting" with it, but it was enough to catch me!!!
> Well, in my research I deal with kernels, flours and baking processes
> and I'd like to better study, also from a microstuctural point of
> view, these materials and the interaction between
> protein-starch-fiber-lipids when the flour is mixed to give dough ...
> but I absolutely ignore how to treat them (sample preparation,
> specific fluorescent labels...) for the CLSM observation. Please,
> would be so kind to give me any help?
> Many thanks in advance,
> Manuela
>
>
> Dr. Manuela Mariotti
> DiSTAM - Food Technology
> University of Milan
> via G. Celoria, 2
> 20133 Milan, Italy
> phone: +39.0250316625
> fax: +39. 0250316632
> e-mail: <mailto:[log in to unmask]>[log in to unmask]
>
>
> Dott. Umberto Fascio
> C.I.M.A.
> Centro Interdipartimentale Microscopia Avanzata
> Via Celoria 26 - 20133 Milano
> tel: +39 0250314807/14876
> fax:+39 0250314802
>
--
Associate Professor Guy Cox
Electron Microscope Unit,
University of Sydney,
NSW 2006, Australia
Phone:+61 2 9351 3176 Fax:+61 2 9351 7682
http://www.guycox.net
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