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Hi Juan,

Don't know which is best for trypanosomas, but try sytox red, toto-3,
to-pro-3 all from Invitrogen, approx 10nm blue shifted em compared to
Cy5. (no commercial interest).

regards, jens
 
---
Dr. Jens Rietdorf 
head of microscopy unit
Friedrich-Miescher-Institute, wro1066.2.32
Maulbeerstr.66, CH-4058 Basel, Switzerland
phone +41(61)69-75172 mobil +41 798284737

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Michael Weber
Sent: Mittwoch, 22. November 2006 15:58
To: [log in to unmask]
Subject: Re: Staining DNA in Cy5 range

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Hi Juan,

DRAQ5 could match your purpose:

http://www.vincibiochem.it/DRAQ5.htm

cheers,
Michael


Juan Luis Ribas schrieb:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello everybody, I'm interested in staining nuclei of trypanosomas 
> with some substance similar to DAPI but that works in the range of 
> Cy5. This is because my confocal doesn't have diode 405 or UV laser. 
> If someone could help me I would be very greatfull.
> Best regards.
> 
> Juan Luis