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I'm not so sure about that. Action potentials can be gotten from dead 
neurons. I worked in a lab once doing EM for a bunch of 
neurophysiologists, and they routinely got action potentials not only 
from dead brain slices, but also slilces where the neurons had been 
dead long enough to lose their membranes. (Needless to say, they 
never liked being told this.)

But, I think there's a typo here. Tino refers to "slice preparation", 
which is a tissue sample thick enough to contain living, intact 
cells, not to "slide preparation".

Phil


>Yes, but I don't quite understand what Tino means by
>slide preparations.  You'll only get a measure of
>membrane potential is the cells are intact and alive.
>
>					Guy
>
>moreno wrote:
>
>>  yes,
>>  DI8-ANNEPS for example.
>>  Tino Jaeger wrote:
>>>  Hi,
>>>
>>>  is there any fluorescence dye, which can can be used to code for the
>>>  membrane potential of cells (e.g. loading of the cells like with FURA-AM,
>>>  and the then fluorescence intensity of the dye shows up the different
>>>  voltage (membrane potential) of cells in a slice preparation)?
>>>
>>>  Thanks for your help.
>>>  Tino
>>>
>>
>
>--
>______________________________________________
>Associate Professor Guy Cox, MA, DPhil(Oxon)
>Electron Microscope Unit, Madsen Building F09,
>University of Sydney, NSW 2006
>______________________________________________
>Phone +61 2 9351 3176     Fax +61 2 9351 7682
>Mobile 0413 281 861
>______________________________________________
>http://www.guycox.net

-- 
Philip Oshel
Microscopy Facility Supervisor
Biology Department
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
voice: (989) 774-3576
dept. fax: (989) 774-3462