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We looked at drift on a Leica confocal, it is unlikely that they differ greatly- it bounced up an down along with the air conditioning.
There is also drift in the X and Y axes
J. Adler and S.N. Pagakis
Reducing Image Distortions Due To Temperature Related Microscope Stage Drift
J. Microscopy, 210, May 2003, 131-137
Jeremy Adler
Cell Biology
The Wenner-Gren Inst.
Arrhenius Laboratories E5
Stockholm University
Stockholm 106 91
Sweden
________________________________
From: Confocal Microscopy List on behalf of Dr. Olaf Selchow
Sent: Tue 14/11/2006 21:12
To: [log in to unmask]
Subject: Thermal Focus Drift - Olympus BX 51 WI -
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Hello List,
anybody out there who has an idea about how much focus shift
(approximately) I can expect from a 5 (10) deg celsius temperature
change in the lab on an Olympus BX 51 WI?
With aproximately I mean: is it 1, 5 or 10 µm?? Or even more?
I know I should keep the temperature constant (the goal in our case is
plus minus 1 deg C) but we need to know how much the focus shifts to
have good arguments to ask for better air conditioning the lab... A
measurement would take time - therefore it would help a lot if someone
has done the test already and can help out with his experience.
Thanks very much!
Olaf
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