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January 2007

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Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 29 Jan 2007 11:10:34 +0100
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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Nucleic Acid Stains for Live Cells
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From:
Maria Calvo <[log in to unmask]>
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Thank you for advice!

George McNamara wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Maria,
>
> SYBR Green I looks good. Stains both nuclei and mitochondrial DNA. 
> Speaking of the latter, todays factoid: I learned from a colleague 
> that mitochondria DNA is in nucleoids of ~5 copies (not single copy as 
> I had assumed).
>
> See Figure 4 of D.H. Margineantu et al 2002 Mitochondrion 1: 425-435 
> (you might try lower concentrations than they used) and C. Briggs , M. 
> Jones 2005 Acta Histochmica 107: 301-312.
>
> Good imaging,
>
>
> George
>
>
> At 05:52 AM 1/24/2007, you wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi,
>> I would like to know what are the best nucleic acid stain for live 
>> cells in the visible range (458-647nm). We want to make long term 
>> experiments, more than 6 hours, and we are looking for a bright (not 
>> bleaching) and not toxic DNA stain. I have read SYTO dyes can be 
>> toxic for cells. What's your experience on DRAQ5 or ethidium bromide 
>> (dihydroethidium)?
>> Thanks all for your help,
>> Maria Calvo
>>
>> Maria Calvo, PhD
>>
>> Unitat de Microscòpia Confocal
>> Serveis Cientificotècnics-C.Casanova
>> Facultat de Medicina-UB
>> Casanova 143
>> Barcelona 08036
>>
>> Tel: 34 934037159/39930
>> Fax: 34 934039946
>> E-mail: [log in to unmask]
>>
>> -- 
>>
>>
>> ___________________________________
>>
>> Maria Calvo, PhD
>>
>> Unitat de Microscòpia Confocal
>> Serveis Cientificotècnics-C.Casanova
>> Facultat de Medicina-UB
>> Casanova 143
>> Barcelona 08036
>>
>> Tel: 34 934037159/39930
>> Fax: 34 934039946
>> E-mail: [log in to unmask]
>> ___________________________________
>
>
>
>
>
>
>
>
> George McNamara, Ph.D.
> UCDMC Stem Cell Program
> Sacramento, CA
> 916-429-7629
> [log in to unmask]
>


-- 


___________________________________

Maria Calvo, PhD

Unitat de Microscòpia Confocal
Serveis Cientificotècnics-C.Casanova
Facultat de Medicina-UB
Casanova 143
Barcelona 08036

Tel: 34 934037159/39930
Fax: 34 934039946
E-mail: [log in to unmask]
___________________________________

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