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hello
We would like to compare the two instruments as we were looking at
writing a grant for a LIVE.
Vickery Trinkaus-Randall
Biochemistry
Boston University School of Medicine
David Knecht wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> In response to Jens's comments, we have been working with actin
> binding protein indirect labeling of F-actin for many years (I think
> we were the first to do it: Pang, et al. (1998) Current Biology
> 8:405) and we are about to submit a paper to show that some, but not
> all probes do in fact alter actin dynamics.
> As to spinning disk, our recent tests show the recent iteration of
> the Zeiss 5Live to be as good as, and in some way better than
> spinning disk for maintaining viability of cells and for speed for 2
> channel imaging. Anyone else done this comparison? Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
> On Jan 3, 2007, at 4:10 AM, Rietdorf, Jens wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear Mark and others,
>> (happy new year!)
>>
>> Be aware, fp-fusions change cytoskeleton dynamics (at least for
>> tubulin). We compared growth and shrinkage rates to other labeling
>> methods.
>>
>> Colombelli J, Reynaud EG, Rietdorf J, Pepperkok R, Stelzer EH. In
>> vivo selective cytoskeleton dynamics quantification in interphase
>> cells induced by pulsed ultraviolet laser nanosurgery. Traffic. 2005
>> Dec;6(12):1093-102.
>>
>> The protein used to label actin (mentioned in other postings) to my
>> knowledge is a construct consisting of lim domains that bind to and
>> thereby indirectly label actin filaments. It would be interesting to
>> see if binding of this construct to the filaments changes actin
>> dynamics.
>> Fischer, M., Haase, I., Simmeth, E., Gerisch, G. and MuĻ ller-
>> Taubenberger, A. (2004) A brilliant monomeric red fluorescent
>> protein to visualize cytoskeleton dynamics in Dictyostelium. FEBS
>> Lett. 577, 227-232.
>>
>> To improve visualisation, TIRF is the imaging method of choice for
>> adherent cells. Otherwise use Yokogawa disk.
>>
>> regards, jens
>>
>> ---
>> Dr. Jens Rietdorf
>> head of microscopy unit
>> Friedrich-Miescher-Institute, wro1066.2.32
>> Maulbeerstr.66, CH-4058 Basel, Switzerland
>> phone +41(61)69-75172 mobil +41 798284737
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of Marc Thibault
>> Sent: Mittwoch, 20. Dezember 2006 22:23
>> To: [log in to unmask]
>> Subject: live cell
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hello all,
>>
>> Does anyone one know of commercially available GFP (or other FP's)
>> tagged cells, with a preference for cytoskeletal tagged proteins ?
>>
>> Thanks
>> Marc
>
>
>
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