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Another trick, incubate in 10mg/ml  polyethyleneimine over night this
might work 
Better than polylysine. 
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Greta Glover
Sent: Saturday, February 17, 2007 2:10 PM
To: [log in to unmask]
Subject: Re: beads in water?

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I had a similar problem recently when trying to acquire a psf for a
water dipping lens- I incubated glass slides in polylysine overnight and
this did the trick, beads stayed still!

Greta Glover
Postdoctoral fellow, Nicolson lab
Oregon Health and Sciences University
3181 SW Sam Jackson Park Rd.
Portland, OR 97239

>>> [log in to unmask] 02/17/07 7:57 AM >>>
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Cell-Tak (BD Biosciences) should work. It will hold live Ciliates in
place; it should hold your beads. Spread the material on your coverslip
while it dries to get a good distribution.

 

No commercial interest.

 

Wade

 

Wade Bell, Ph.D.

Associate Professor of Biology

Virginia Military Institute

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________________________________

From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Jennifer Waters
Sent: Saturday, February 17, 2007 10:01 AM
To: [log in to unmask]
Subject: beads in water?

 

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Hi all,

 

I'm trying to stick 6 micron polystyrene beads to a glass coverslip and
mount them in water.  I'm drying the beads onto the coverslip, but they
wash away when I add the water.  We do this all the time mounting them
in glycerol and it works fine.  Any suggestions on how to get the beads
to stick? 

Thanks, Jennifer

--
Jennifer Waters, Ph.D.
Director, Nikon Imaging Center at Harvard Medical School