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Hello,
      Can anyone tell me how long one can store mammalian cells in a -80
freezer without the use of liquid nitrogen.

We are looking for a short term storage (3-6 months) for continuous use of
primary cells and cells lines, but don't have access to liquid nitrogen
storage. Hence this solution of freezing batches in  -80 freezer.

Also does it make a difference using whether a -80 or a -86 freezer.


Thanks,

-Prabhakar