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Hello Claire -
 
Maybe you can try defocusing to see if the cells stand out better and can be segmentated by brightness. - 
 
Mike Model

________________________________

From: Confocal Microscopy List on behalf of Claire Brown
Sent: Wed 5/2/2007 9:56 AM
To: [log in to unmask]
Subject: Counting Cells in Brightfield


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I have a user interested in using metamorph to count cells in DIC/brightfield. There is not a lot of contrast between the cells and the background. How do people manage to count with these kinds of images?
 
Sincerely,
 
Claire
 
 

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Claire M. Brown, PhD

Life Sciences Complex Imaging Facility Director

McGill University Department of Biochemistry

http://www.lifesciencescomplex.mcgill.ca/imaging <http://www.lifesciencescomplex.mcgill.ca/imaging>  <http://www.lifesciencescomplex.mcgill.ca/>