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Hi,
     We have a custom-made two-photon microscope in our lab. When I 
construct it I ordered a Hamamatsu PMT module (HC120-13 MOD) with an 
integrated amplifier of 10MHz on it. Since now we are collecting image 
with 512x512 @1fps (260KHz sampling rate), I think the amplifier just 
"eat" a lot of my signal. Now the question: what is the proper 
amplification frequency for my application? Exactly equal to sampling 
rate or I need to consider Shannon-Nyquist sampling theorem (2x the 
sampling rate) or something else?
    Thank you!

Sincerely,
Peng Xi
Dantus Research Group
Department of Chemistry
Michigan State University
East Lansing, MI 48824
Tel: (517) 355-9715 x319
Email: [log in to unmask]
http://www.msu.edu/~xipeng/