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Hello,

This will not answer directly, but how about lubricating the threads?

Regards,
Sophie
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Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
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Quoting F Javier Diez Guerra <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We have a Coolsnap FX mono behind a Sutter
> lambda-10 emission filter wheel, that is  used for epifluorescence
> microscopy.
>
>  From time to time I check illumination flatness,
> alignments, etc. However, when I look at
> transmission samples using brightfield or
> phase-contrast I notice small particles of dust
> located always at same position of the image.
>
> I have examined the microscope and the dust seems
> to be located on the glass in front of the CCD of
> the camera. Careful cleaning with anti-static
> clothe and air-stream only changes the distribution of the dust particles.
>
> After repeating several times the same operation,
> I have realized that the dust comes from small
> particles released in the process of screwing the
> camera into the filter wheel thread.
>
> Has anybody experienced something similar? Any
> recommendation to keep clean the CCD window?
>
> Thanks,
>
>
> F Javier Diez-Guerra, PhD
> Profesor Titular
> Centro de Biologia Molecular Severo Ochoa
> Facultad de Ciencias, Universidad Autónoma
> Ctra Colmenar Viejo Km 15
> Cantoblanco, 28049 Madrid
> SPAIN
>
> phone:  +34 91 4978051
> Fax:      +34 91 4978087
> e-mail: [log in to unmask]
>