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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
For shorter term experiments (up to 24 hours) I often use the base from a
Petri dish, with a hole cut through the centre so as not to interfere with
the light, then add in a piece of damp filter paper, with an equialent hole
cut through it. I then invert this over the sample and it helps prevent my
agar block and liquid cultures from drying out. If the height is an issue
you can get deeper and wider Petri dishes.
Good luck,
Graham
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Marc Thibault
Sent: 21 June 2007 20:37
To: [log in to unmask]
Subject: Re: live cell imaging media evap problem
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Similarly, when space is limited, we put 3-4 inverted falcon tube caps
filled with water.
MArc
------------------------------------------------
Marc Thibault, Ph.D.
Postdoctoral Fellow
NSERC/Biosynthech Chair in Hybrid Biomaterials for Innovative Regenerative
Technologies Department of Chemical Engineering Ecole Polytechnique
Montreal, Qc, Canada
514 340 4711 (4781)
------------------------------------------------
-----Message d'origine-----
De : Andrew Resnick [mailto:[log in to unmask]] Envoyé : 21 juin 2007
07:52 À : [log in to unmask] Objet : Re: live cell imaging media
evap problem
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We routinely do overnight incubations, and I'm trying to extend that into
weekend-long incubations. The simple trick that helped us was to simply put
a small beaker of water directly onto the stage. The stage is slightly
warmer than the surroundings due to the motors, and so the water will
evaporate and supply humidity for the surroundings.
Andy
At 05:07 PM 6/20/2007, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi There,
>I was wondering what people are using to avoid media evaporation during
>live cell imaging (long time courses - 24-48 hours / in 8 well labtek
>chambered coverslips). We have a home made temperature control
>incubator surrounding our LSM 510 NLO with 2 photon. We cant use
>perfusion setup because expensive drugs are being pipetted in each well
>throughout the time course. The lid of the chambered coverslip seems
>to help, but it causes the issue of if while removing the lid to add a
>drug in the middle of the experiment, it knocks anything even the
>slightest bit, the entire xy and z marked positions are completely
>thrown
off.
>Any suggestion are welcome.
>
>-Surita
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Surita Banwait
>Morphology & Imaging Core
>Buck Institute for Age Research
>8001 Redwood Blvd.
>Novato, CA 94945
>415-209-2221
>[log in to unmask]
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[log in to unmask]]
>On Behalf Of Andrew M. O'Grady
>Sent: Wednesday, June 20, 2007 2:50 PM
>To: [log in to unmask]
>Subject: Problems with lamp housing for OSRAM 103HBO
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello Everyone,
>
> This is my first email to this list. I apologize that this is not
>exactly related to microscopy, but this seems to be the largest
>knowledge base about mercury vapor lamps that I have been able to find
>on the internet.
> We are using an HBO 103W bulb for our Schlieren imaging system for
>supersonic flow visualization. The old setup used an OSRAM 200W/2 bulb
>with a lamp housing and lens (J. Unertl Optical Co.) and a power supply
>from Robert W. Gates & co. The equipment must be at least 30 years
>old, if not 50 years. Recently the power supply stopped working, and we
>purchased a Nikon C-SHP1 power supply. We hooked up the power supply to
>the old housing (making certain modifications), and used an HBO103W
>bulb which worked fantastically for about 30 hours, having been turned
>off and on maybe 10 times.
>The last time I tried to light it, the "Lamp Ready" light flickered for
>1 or 2 seconds and then went off. Examining the lamp, it did not
>explode, but the inside of the glass is covered with mercury, so that
>the electrodes are not even visible. I did not even try to relight the
>bulb, I just assumed it was ruined.
>
>I think that the bulb has probably failed from the modifications that
>we made to the connections in the housing. The first modification was
>that the mount for the bottom of the bulb had to be made smaller
>because of the smaller diameter of the 103Wbulb.
>This we did by using a small sleeve of copper inserted into whatever
>metal material the existing mount was, possibly bronze.
>After the bulb stopped working, the inside of the copper sleeve is
>tinged pink, compared to the outside.
>
> For the top bulb mount, the old style bulb we were using had a screw
>on it, and was attached to a flexible wire protected by ceramic beads.
>The new style does not have a screw, so we made another sleeve out of
>copper with a screw to tighten. The flexible wiring with ceramic is
>still used to account for thermal expansion.
>After running, the top copper mount is now dark gray-colored (probably
>oxide), but this can be scratched off.
>
>One other thing I noticed before the bulb would not light was that
>there appeared to be some small bubbles inside of the bulb near the
>bottom electrode after I had run it a few times.
>
>I appreciate people taking the time to help me on this, and I intend on
>attempting to contact OSRAM regarding this matter, but this seemed like
>a good place to look for assistance.
>
>Thanks,
>Andrew O'Grady
>
>--
>Andrew M. O'Grady
>212-854-7306
>PhD Candidate
>Mechanical Engineering Department
>Columbia University
>Mudd Building
>500 West 120th Street
>New York, NY 10027 USA
Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)
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