CONFOCALMICROSCOPY Archives

June 2007

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From:
Tom Blanpied <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 22 Jun 2007 10:29:33 -0400
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi,

The LED technology is certainly filling a more and more broad set of roles 
these days, and resistance to power surges is a useful benefit.  Slightly 
more specifically, has anyone tested the LED arrays for photoactivation? 
The spectrum of the UV bulb should be perfect for PA-GFP, and I'd be 
curious how it compares to other non-laser sources. 

Gerry, does the company have available a direct comparison of spectral 
irradiance of the LAMs vs XBO (or metal halide) lamps?  

Tom
================================================
Thomas A. Blanpied, Ph.D.
   Department of Physiology
   University of Maryland, Baltimore
   516 Howard Hall, 660 W. Redwood St.
   Baltimore, MD 21201
   http://physiology.umaryland.edu/faculty/TBlanpied/
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   Tel (410) 706-4769
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On Thu, 21 Jun 2007 13:00:51 -0400, Gerard Whoriskey <Gerard.Whoriskey@CIL-
UK.CO.UK> wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Technical response:
>
>Thank you Russell for pointing out the suitability of the precisExcite 
>from CoolLED for time lapse work. You are absolutely right!  
>Being a fully solid state LED illumination system (no mercury issues) the 
>product does not suffer from the many problems of arc lamps. 
>For time lapse experiments the precisExcite can be operated through 
>software or TTL pulses, to switch on then off only when a picture needs 
to 
>be taken. There is no need to leave the device on constantly like a bulb, 
>also no need for shutters. A particular advantage for time lapse work is 
>its stability, in intensity and homogeneity over time. 

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