Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Sam,

Thanks for all of that information - very helpful.

Ray



Wadsworth Samuel wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi Ray,
>
>We're using verapamil hydrochloride (Sigma 381195, 50microM) which seems
>to block hoechst efflux pretty well in our cells.  
>
>We also use Fumitremorgin C (Sigma F9054)...Sigma says; "Fumitremorgin C
>is a specific, selective, and potent inhibitor at micromolar
>concentrations of the breast cancer resistant protein (BCRP/ABCG2), an
>ABC transporter associated with chemotherapy resistance. FTC, in
>combination with mitoxantrone, can be used for the detection of ABCG2
>functional activity in several cell lines.".
>
>Good luck
>
>Sam
>
>Sam Wadsworth PhD
>Division of Therapeutics & Molecular Medicine
>D Floor, South Block,
>Queen's Medical Centre,
>University Hospital,
>Nottingham 
>NG7 2UH
>
>Tel: 0115 8231070
>Fax: 0115 8231059
>email: [log in to unmask]
>
>
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[log in to unmask]] On
>Behalf Of Ray Hester
>Sent: 07 June 2007 20:15
>To: [log in to unmask]
>Subject: which form of verapamil?
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Can anyone tell us which one of the four forms of verapamil that
>Sigma-Aldrich offers (below) we should add to cell cultures, in which we
>are using Hoechst 33342 to stain for ploidy/cell cycle, in order to
>stop/lessen the efflux of Hoechst that occurs in some cells.
>
>hydrochloride
>hydrochloride hydrate
>monohydrochloride monohydrate
>and, methoxyverapamil hydrochloride
>
>Thanks.
>
>Ray Hester
>Univ of South Alabama
>
>This message has been checked for viruses but the contents of an attachment
>may still contain software viruses, which could damage your computer system:
>you are advised to perform your own checks. Email communications with the
>University of Nottingham may be monitored as permitted by UK legislation.
>  
>