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Criag
If spectral analysis is the endpoint you may want to consider the PARISS
system from Lightform. ( http://www.lightforminc.com/) that can be
placed on a widefield microscope.
We are using the PARISS system to obtain spectral data --it has 1nm
resolution and has a spectrum from 400-900nm. I find it to be a great
asset in the laboratory,
It is far more sensitive and accurate that any confocal system that I
have seen.
Contact Jeremy Lerner for details on his system.
best wishes
Bob
LightForm, Inc.,
601 Route 206, Suite 26-479
Hillsborough NJ 08844
Tel: (908)281 9098
Email: [log in to unmask]
.
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Telephone: 919-541-1585 Fax: 919-541-4017
e-mail: [log in to unmask]
Mail address: Reproductive Toxicology Division, MD 67
2525 E.NC Highway 54
Research Triangle Park, North Carolina, 27711
Shipping address: 2525 E.NC Highway 54
Durham, NC, 27713
Craig Brideau
<craig.brideau@G
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<CONFOCAL@LISTSE Subject
RV.BUFFALO.EDU> Re: Nikon C1si?
10/05/2007 05:48
PM
Please respond
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Thanks for your input! As in everything, there's a trade-off. Still,
for us the spectral data will be worth it.
Craig
On 10/5/07, Robert Zucker <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Craig
> The efficiency of detection is much less in the spectral mode due to
the
> design of the multianode detector. In addition as Kurt Thorn said the
> light is split into a number of different bandpass channels instead of
> being detected by only one channel which also limits the detection in
> each channel.
> Nikon compensates for this decreased light by increasing the pixel
dwell
> time. However photons are photons. With less photons you will get a
> noisier image.
> The spectral detection of any confocal system using a multianode
> detector will not be as good as a PMT designed for good sensitivity
and
> low light detection. It will produce nosier images but if there is
> enough light you will be able to determine a valuable spectrum which
can
> be used for scientific experiments and to manipulate images. Like
> everything with confocal microscopy there is trade-offs and no perfect
> system.
> Best wishes.
> Bob
>
> Robert M. Zucker, PhD
> U.S. Environmental Protection Agency
> Office of Research and Development
> National Health and Environmental Effects Research Laboratory.
> Telephone: 919-541-1585 Fax: 919-541-4017
> e-mail: [log in to unmask]
>
> Mail address: Reproductive Toxicology Division, MD 67
> 2525 E.NC Highway 54
> Research Triangle Park, North Carolina, 27711
>
> Shipping address: 2525 E.NC Highway 54
> Durham, NC, 27713
>
>
>
>
> Craig Brideau
> <craig.brideau@G
> MAIL.COM>
To
> Sent by: [log in to unmask]
> Confocal
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> Microscopy List
> <CONFOCAL@LISTSE
Subject
> RV.BUFFALO.EDU> Re: Nikon C1si?
>
>
> 10/04/2007 02:49
> PM
>
>
> Please respond
> to
> Confocal
> Microscopy List
> <CONFOCAL@LISTSE
> RV.BUFFALO.EDU>
>
>
>
>
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I was wondering if you saw this decreased throughput in spectral mode
> only, or also in conventional detection mode? The spectral mode works
> with a multi-anode PMT, which has a lower quantum efficiency than a
> conventional PMT. From what I have seen of the design, it also has
> the option to use a conventional PMT system with the device, and I am
> wondering if you have also tried that mode of operation?
>
> Thanks,
>
> Craig
>
> On 10/4/07, Robert Zucker <[log in to unmask]> wrote:
> > One of the major limitations that we have seen with this unit and
also
> > with the Zeiss meta 510 is the decreased light throughput . This
> > creates images that are noisier than conventional confocal
> microscopes.
> > In our hands it appears you will need to have a bright sample to
make
> > the spectral system work properly. ,
>
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