Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi David,

We have no trouble with our SP2, except with a LWD 40 dry objective.  
I think the issue of gradients is often one of a mismatch between the 
Wollaston prism and the objective.

Also, check the settings of your condenser aperture.  On our system, 
that aperture has a profound effect on the DIC images.

I find that there is greater fluctuation with our Argon laser than 
with the HeNe green and red lasers.  Since we often use sequential 
scan, I do the DIC image with the green laser source.

Joel


-------------- Original message ---------------
Date sent:	Wed, 31 Oct 2007 08:26:44 -0400
Send reply to:	Confocal Microscopy List 
<[log in to unmask]>
From:	David Knecht <[log in to unmask]>
Subject:	Re: transmited ligth and LSM
To:	[log in to unmask]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-
bin/wa?S1=confocal We also have problems with DIC on our SP2 which 
have never been fixed. In our case it is sometimes rings of light 
dark, and sometimes serious gradients across the field.  Dave 
Dr. David Knecht  
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)



On Oct 31, 2007, at 6:52 AM, Rosemary White wrote:





Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [log in to unmask]
URL:  http://astro.temple.edu/~jbs