Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

not if you look at an almost vertical feature, e.g., the side of a sphere 
(or a pollen grain)

Stan 

On Wed, 30 Jan 2008 22:51:40 -0500, George McNamara 
<[log in to unmask]> wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Reflected light confocal microscopy is much brighter than using a
>fluorescent dye.
>
>
>
>
>At 07:02 PM 1/30/2008, you wrote:
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Dear confocalists,
>>do you have suggestions for a general counterstain that would fluoresce 
in
>>green?
>>I am doing 3D imaging of large number of pollen samples, and would like 
to
>>increase the signal levels so that my acquisition times become more
>>reasonable.
>>
>>The pollen has been processed (cytoplasm removed), so I am trying to 
stain
>>the shell (exine/intine) and am not interested in the cytoplasm.
>>
>>Rhodamine B staining worked fine, but the emission is in red. I am after
>>shorter wavelength signal for best resolution.
>>
>>Thank you!
>>
>>
>>Stan Vitha
>
>
>
>
>
>
>George McNamara, Ph.D.
>University of Miami, Miller School of Medicine
>Image Core
>Miami, FL 33010
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>305-243-8436 office
>http://home.earthlink.net/~pubspectra/
>http://home.earthlink.net/~geomcnamara/
>http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc (see
>Analytical Imaging Core Facility)
>=========================================================================