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Hi, Peng Xi

The big issue is going to be the transmission of any of the optics, 
from the fiber optic through the microscope (including prisms and 
objectives). Most glassware drops off abruptly somewhere between 360 
and 380.  My best advice: have a long chat with Nikon tech support.

Hope this was helpful,

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At 04:53 PM 2/3/2008, Peng Xi wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi,
>     Has anyone have the experience of delivering 350nm laser in to a
>microscope? I'm planning to use this line for autofluorescence
>excitation with a Nikon microscope, I'll use the side-port to deliver
>the excitation to construct a custom-made confocal setup, but I don't
>know the absorption ratio. Currently I'm worrying about the absorption
>from the reflective prism; if the absorption is very large I'll
>consider to use the back port.
>     Thank you for any input!
>
>Best,
>Peng Xi
>Associate Professor
>Institute for Laser Medicine and Biophotonics
>Shanghai Jiao Tong University
>800 Dongchuan Rd.
>Shanghai 200240, China
>Tel: (86) 21-3420-4076
>http://biophotonics.sjtu.edu.cn/