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Hi Hanspeter,
You  might consider assessing filter characteristics with a scanning 
spectrophotometer.  Assuming you have access to one, you simply place the 
filter in the light path and have the scanner produces a transmissiton vs. 
wavelength plot.  It produces a very nice detailed spectral plot that would 
be comparable for all components analyzed.  Repositioning the filter will 
give you an idea of field uniformity.

Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
----- Original Message ----- 
From: "Hanspeter Niederstrasser" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, February 01, 2008 11:26 AM
Subject: fluorescent reference slides/beads for spinning disc


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We're testing new probes and filters for our microscope and amongst the 
> things we wanted to check was signal loss btw different filters as well as 
> intensity distribution across the view field (this is separate from the 
> filters of course).  I found Invitrogen's FluoSpheres as well as Fluor-Ref 
> fluorescent slides (Ted Pella being one vendor here: 
> <http://www.tedpella.com/histo_html/fluor.htm>).
>
> The reference slides are obviously much more economical, but they don't 
> list their emission spectra, and since the filters we're testing have 
> fairly different OD spectra, we would like the references to be as similar 
> to our probes.  What are people's experiences and recommendations with 
> these references or others you have used?
>
> Hanspeter
>
> -- 
> Hanspeter Niederstrasser, Ph.D.      Dept. of Microbiology
> hn2157 at columbia dot edu           701 W. 168th St.
> Chang Lab                            New York, NY 10032
> Columbia University
>