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Hi, Glen,

The picture is from one of our users.  It was taken somewhere else in the past 
and the user would like to do the counting in our lab at this stage.  I have 
no knowledge about the lablel and mountant.  But I believe widefield was used 
and it was a single plane image, and the tissue was dog retina, likely whole 
mount.  The objects here are cross-section view of the inner segments of the 
rod cells.  Obviously it was not a good quality picture to start with, but 
this is all he has got.  

Thank you very much for your attention.

Xinyu 



Quoting Glen MacDonald <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Are you using confocal or widefield, was that image a single plane or  
> a brightest point projection? what is the label and mountant?
> 
> Glen
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923  USA
> (206) 616-4156
> [log in to unmask]
> 
> ************************************************************************ 
> ******
> The box said "Requires WindowsXP or better", so I bought a Macintosh.
> ************************************************************************ 
> ******
> 
> 
> On Feb 6, 2008, at 12:28 PM, Xinyu Zhao wrote:
> 
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >
> >
> > Dear listers,
> >
> >  I was wondering if anybody could give me some advice on how to  
> > count the
> > objects in the image as attached.  They were cross sections of  
> > inner segments
> > of rods and the goal is to get the total number of them.
> >
> > It seemed a easy job at the beginning.  But after I started, I  
> > realizee that it
> > is tricky.  The intensity variation of the objects is big and the  
> > boundaries of
> > the cells are not clear.
> >
> > I am not much of an image processing person.  I was wondering if  
> > anybody could
> > give me some advice on how to proceed.
> >
> > Thank you very much.
> >
> > Xinyu Zhao
> > Biomedical Imaging Core Lab
> > B110 Richards Building
> > School of Medicine
> > University of Pennsylvania
> > 37th and Spruce Street
> > Philadelphia, PA 19104<#1978, ODA, 1,000um from ONH, 40X.tif>
> 
> 


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