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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi,

I was not intending to make it international but feel free to show up.
_______________________________________________________________________
The Cell Imaging and BioInformatics Units are organizing a course about
Quantitative image processing. It will be held at the IGC on the
bioiformatics computer room in 26 and 27 February.

Synopsis:
Imaging analysis, though very powerful, its also a subject of several
artifacts which have to be taken in account on experimental design and
acquisition. Notwithstanding, it is the desirable method for taking
information in microscopy, making us focus on the need of this approach,
acquisition details and results obtained within this scope. Because it
will be "problem oriented", applicants should propose a real or putative
problem for the practicals, which will be the most important selection
factor.

Instructors:
Nuno Moreno (Cell Imaging Unit-IGC) Course Organizer
José Leal (BioInformatics Unit-IGC)
Mónica Dias (Cell Cycle Regulation-IGC)
Gabriel Martins (Centro de Biologia Ambiental-FCUL)


For applying please visit:
http://bioinformatics.igc.gulbenkian.pt/events/39/

This course is restricted to 20 people and its free for IGC/ITQB
members, having a symbolic fee of 100 Euros for others.


See you there,
Nuno Moreno

Marius Messerli wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> - commercial response -
> 
> Dear  Xinyu,
> 
> Imaris determined a count of 1859.
> 
> The procedure:
> - drop image file into Imaris
> - create new spot component
> - step through detection wizzard
> - open statistics
> 
> The Imaris Spot detection/analysis component knows how to handle variable
> image intensity and cell size.
> 
> Let me know if you are interested in the final image (overlay of your
> original image with detected spots) or an evaluation copy of Imaris.
> 
> Regards,
> Marius
> 
> Marius MESSERLI, Ph.D.
> CEO Bitplane
> http://www.bitplane.com
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Xinyu Zhao
> Sent: Mittwoch, 6. Februar 2008 21:29
> To: [log in to unmask]
> Subject: advice on counting objects
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> 
> 
> Dear listers,
> 
>  I was wondering if anybody could give me some advice on how to count the
> objects in the image as attached.  They were cross sections of inner
> segments of rods and the goal is to get the total number of them. 
> 
> It seemed a easy job at the beginning.  But after I started, I realizee that
> it is tricky.  The intensity variation of the objects is big and the
> boundaries of the cells are not clear.  
> 
> I am not much of an image processing person.  I was wondering if anybody
> could give me some advice on how to proceed.
> 
> Thank you very much.
> 
> Xinyu Zhao
> Biomedical Imaging Core Lab
> B110 Richards Building
> School of Medicine
> University of Pennsylvania
> 37th and Spruce Street
> Philadelphia, PA 19104
> 

-- 
Nuno Moreno
Cell Imaging Unit
Instituto Gulbenkian de Ciência
http://uic.igc.gulbekian.pt
http://www.igc.gulbekian.pt
phone +351 214464606
fax   +351 214407970