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Hi Friedrich

Are you sure you are seeing the excitation light 
reflected by the pipet? If so maybe more 
stringent filtering might help with your problem? 
If not, might it  rather be the fluorescence 
emission of your sample that is reflected by the pipet?

BTW, as a general question to the list: is there 
a reason why one couldn't use two inteference 
filters in series? The ODs should simply add up, shouldn't they?

Beat

At 15:13 12-03-2008, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear all,
>I am using a CSU-22 spinning disc for calcium 
>imaging in dendrites and spines of neuronal 
>cells. When doing synaptic stimulation, the 
>glass pipette is positioned pretty close to the 
>imaged region and therefore reflects the 
>excitation light. My question: does anybody have 
>experience with non-reflecting coatings that can 
>be applied to glass pipettes and are of no 
>effect on the tissue? We thought about black 
>nail polish and would like to know if anybody has a betteer suggestion.
>Thanks a lot,
>
>Friedrich
>
>
>Dr. Friedrich W. Johenning
>NWFZ -Campus Mitte / AG Schmitz
>Charite University Medicine Berlin
>Charitéplatz 1
>10117 Berlin
>Tel.: 030 450 528077
>Mobil: 0173 2144701
>Fax: 030 450 539943