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Dear Vaishali,

I'd suggest to check whether your slides  are hydrophobic or 
hydrophylic. Cells usually don't like hydrophobic ("dirty") glass 
slides. A short (oxygen) plasma treatment could help. Of course there 
are also many solvent based cleaning protocols around.

Cheers    Gabor

-- 
Gabor Csucs 
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16 
CH-8093, Zurich, Switzerland

Web: www.lmc.ethz.ch
Phone: +41 44 633 6221
Fax: +41 44 632 1298
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