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Dear Tom,

So,do you think it is a  good approximation of SNR if I find the ratio
of the max photon count to the min one of the PSF graph?

Sarah

On Thu, Apr 10, 2008 at 6:10 PM, Sarah Kefayati <[log in to unmask]> wrote:
> Dear Mike,
>
> Yes,You are right.The reseaon that I use beads is I have embeded beads
> inside my sample so by measuring them in the different depths I can
> have a good scale of being in the focal plane and then measuring the
> SNR.
>
> regards
> sarah
>
>
> On Thu, Apr 10, 2008 at 5:30 PM, MODEL, MICHAEL <[log in to unmask]> wrote:
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Maybe I am misunderstanding your question, but if you are interested in
> > SNR why do you need beads? Cannot you just measure SNR for a comparable
> > signal coming from something larger than a bead?
> >
> > Mike Model
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[log in to unmask]] On
> > Behalf Of Sarah Kefayati
> > Sent: Thursday, April 10, 2008 4:41 PM
> > To: [log in to unmask]
> > Subject: SNR
> >
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >
> > Dear all,
> >
> > I appreciate if you help  me to figure out how I can calculate the SNR
> > from the data that I obtain by imaging the fluorescent beads in
> > different depths.
> >
> > Thanks
> > Sarah
> >
>