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Oh yes, and there is one major piece of glass you can't avoid that's  
in front of the light source... the glass substrate! The only way  
around this is to use special quartz coverslips that transmit the UV,  
but I think they are expensive. Not sure who sells these, but we had  
some of these a long time back. Maybe SPI?

John Oreopoulos

On 14-May-08, at 10:39 AM, Michael Weber wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Glyn,
>
> I doubt that you will get a reasonable signal. The main limitation  
> might be the objective and its transmittance in the range of 300nm,  
> which is typically in the range of 0%. Check their objective database:
>
> https://www.micro-shop.zeiss.com/us/us_en/objektive.php?s=t
>
> Afaik the rest of the optics work more or less for wavelengths  
> above 350nm.
>
> Michael
>
>
> Glyn Nelson wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> Hi,
>> Can anyone tell me if a Zeiss LSM510 on an Axiovert200M with a  
>> standard
>> HBO103 lamphousing has a filter in to cut out UV around 250-300nm?
>> I've a user who would like to look at tryptophan fluorescence in  
>> their IHC
>> slices.  To do so on our confocal, I'd have to utilise the Hg lamp  
>> with a
>> 270/50 excitation filter and suitable dichroic in the reflector  
>> filter cube
>> to the meta head PMTs and obtain a non confocal image over the  
>> range of
>> approx 325-360nm.  I know the image won't look terribly pretty,  
>> but they
>> wish to capture the fluorescence to use it to normalise lipofuscin
>> fluorescence from sample to sample.
>> Aside from the fact that I would have to make sure they didn't  
>> stare down
>> the eyepieces with this filter in place, I'm worried that the  
>> 'scope body or
>> lamp housing has a piece of glass in to cut out these sort of  
>> excitation
>> wavelengths from the lamp (I know the lamp has a mercury spike at  
>> about 250
>> nm) so that you don't fry your samples/eyeballs.
>> Anyone who knows this, or knows where I should look to find out,  
>> I'd be
>> grateful to hear from you (I've tried emailing Zeiss with no reply).
>> Thanks,
>> Glyn.