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Date: | Wed, 18 Jun 2008 10:21:20 -0700 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
When I say external detectors for the SP5 I mean NDDs.
JP
Jean-Pierre CLAMME wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi,
>
> First thank you for the infos.
>
> Second do you need to go back through the scan heads ? Is it possible
> to use another side port to extract the light for example to do cross
> correlation ? I worked on two home made systems (FCS + imaging and
> single molecule) were we would collect the light directly after the
> objective and redirect the signal to multiple APDs. On the SP5 we have
> here we have external detectors. Could that kind of approach be used ?
>
> Thanks
>
> JP
>
> Michael Weber wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Jean-Pierre,
>>
>> on both systems one needs to install the external port modification
>> (X1 / port 4 option), in order to bring the light out of the
>> scan-head. Beside that, it's just another discussion about which
>> system offers better transmission - dichromatic mirrors or AOBS.
>>
>> Gabor, can you control the APDs for imaging via the Leica software,
>> or do you have to use an external solution?
>>
>> Michael
>>
>>
>> Jean-Pierre CLAMME wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> Hi,
>>>
>>> I'm wondering if onyone has experience with modifying a SP5 or a
>>> Fluoview
>>> 1000 for FCS mesures ? I work with Olympus stands before and they
>>> generally
>>> offer good access to external ports. But what a bout the SP5 ?
>>> Thanks
>>>
>>> JP
>>
>>
>
--
Dr. Jean-Pierre CLAMME
Supervisor 2-Photon Imaging Facility
Dept. of Immunology, IMM-1, R310
The Scripps Research Institute
10550 North Torrey Pines Rd.
La Jolla, California 92037
Lab number: (858)784-8184
Fax number:(858)784-9272
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