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June 2008

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From:
Jean-Pierre CLAMME <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 18 Jun 2008 10:21:20 -0700
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

When I say external detectors for the SP5 I mean NDDs.

JP


Jean-Pierre CLAMME wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi,
>
> First thank you for the infos.
>
> Second do you need to go back through the scan heads ? Is it possible 
> to use another side port to extract the light for example to do cross 
> correlation ? I worked on two home made systems (FCS + imaging and 
> single molecule) were we would collect the light directly  after the 
> objective and redirect the signal to multiple APDs. On the SP5 we have 
> here we have external detectors. Could that kind of approach  be used ?
>
> Thanks
>
> JP
>
> Michael Weber wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Jean-Pierre,
>>
>> on both systems one needs to install the external port modification 
>> (X1 / port 4 option), in order to bring the light out of the 
>> scan-head. Beside that, it's just another discussion about which 
>> system offers better transmission - dichromatic mirrors or AOBS.
>>
>> Gabor, can you control the APDs for imaging via the Leica software, 
>> or do you have to use an external solution?
>>
>> Michael
>>
>>
>> Jean-Pierre CLAMME wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> Hi,
>>>
>>> I'm wondering if onyone has experience with modifying a SP5 or a 
>>> Fluoview
>>> 1000 for FCS mesures ? I work with Olympus stands before and they 
>>> generally
>>> offer good access to external ports. But what a bout the SP5 ? 
>>> Thanks
>>>
>>> JP
>>
>>
>

-- 
Dr. Jean-Pierre CLAMME
Supervisor 2-Photon Imaging Facility 
Dept. of Immunology, IMM-1, R310
The Scripps Research Institute
10550 North Torrey Pines Rd.
La Jolla, California 92037

Lab number: (858)784-8184
Fax number:(858)784-9272

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