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You can not save the curve directly but you can export it and save the curve.
ML



At 01:23 PM 7/24/08, you wrote:
>Search the CONFOCAL archive at
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>
>Dear Yi,
>
>You cannot directly save the curve -except you want to work on a
>screenshot- but you can select to show a table containing the plotted
>values, these values can be exported to another software to regenerate
>the graph.
>
>There is a 'ROI' folder somewhere in the AIM tree containing the
>position coordinates of the ROIs you generated. To read data from the
>bleached region, you can recall the ROI from a list as described by
>Leoncio.
>
>Just as a FRAP aside: be careful with the bleaching power. You can
>easily achieve powers sufficient to influence molecular interactions
>with a standard 15mW laser...
>
>Cheers, jens
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[log in to unmask]] On
>Behalf Of Vergara, Leoncio A.
>Sent: Wednesday, July 23, 2008 9:35 PM
>To: [log in to unmask]
>Subject: Re: Question about LSM510 software
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I am not sure if there is a way to save both the curve and the images,
>although it would be nice and useful to see the curve in real time, I
>usually do the FRAP experiments in our LSM510 meta just saving the
>images, I look at the curve off line, its very easy to reload the images
>and use the MeanROI to get the curve from the saved images.
>
>regarding savin g the ROI you used for bleaching, there is an option to
>save the ROI from the ROI editing window. You can always retrieve it
>later when using the Mean ROI function... just look at the ROI listing.
>
>Leoncio
>
>
>
>________________________________________
>From: Confocal Microscopy List [[log in to unmask]] On Behalf
>Of Yi Zheng [[log in to unmask]]
>Sent: Wednesday, July 23, 2008 1:39 PM
>To: [log in to unmask]
>Subject: Question about LSM510 software
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear all,
>
>I am practice FRAP on Zeiss LSM 510-META.
>
>Right now I do know how to induce the bleach and record the recovery
>curve
>of ROI(by using click "MeanROI" in time series control ). But everytime
>at the
>end of recording, only the curves are saved. So my question is can I
>save the
>series of image data as well as the  curve?
>
>Another question is after induce bleach I need to restart the scanning
>and
>draw the ROI to measure the mean intensity of them. I always worry about
>that I might miss the ROI where bleaching happens. Does anyone here know
>how to do it in a proper way?
>
>I really appreciate any help!
>
>Thanks,
>
>
>Yi