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Sorry, I forgot to add to my post earlier in the day "no commercial  
interest" as well. I just happened to pick two Olympus examples there  
as well.

John Oreopoulos


On 24-Jul-08, at 3:50 PM, Rietdorf, Jens wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Vitaly,
>
> Both Leica and Zeiss (no commercial interest) provide solutions for
> automatic adjustment of the incident beam angle (provided you measure
> the tilt of the reflecting interface for each sample). We used simple
> stepper motors to automate the turning of the micrometer screw of an
> Olympus 3 line setup (again n.c.i) Be aware, the field is decaying
> exponentially and you can influence for example the half evanescence
> field depth by the angle, [so the distance at which the incident power
> has decreased to 50%], but not the depth of the field of view,  
> which in
> turn also depends on the incident power and on the concentration and
> brightness of the fluorophores.
>
> Cheers, jens
>
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[log in to unmask]] On
> Behalf Of Vitaly Boyko
> Sent: Thursday, July 24, 2008 4:48 PM
> To: [log in to unmask]
> Subject: TIR critical angle re-adjustent
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear All,
>
> Yesterday I have tested our new 585 nm laser in conjuction with 514 nm
> argon
> and 437 nm diode under TIR conditions.
>
> Even though I have not noticed the need for the critical angle
> re-adjustment
> between 437 nm and 514 nm lines (77 nm difference), however, when the
> critical angle was set for the yellow laser (585 nm), switching  
> from 585
> nm
> to 514 nm line  (71 nm difference) required the (manual) resetting of
> the
> critical angle.
> I used the 89006 triple beamsplitter (A/R coated), which seemed to
> reflect
> the 514 argon line (up to 70%).
>
> I am looking for help with the device that would allow quick, low msec
> range, critical angle re-adjustment or any other suggestions will be
> appreciated.
>
> Thanks a lot in advance,
>
> Vitaly
>
> Vitaly P. Boyko,
> NCI-Frederick,
> 301-846-6575
>
>
>