Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi, Yi - there is a well-hidden setting in the Options --> Settings Menu.
Look for "Scan Mean of ROIs" - there are check boxes for scanning and saving
the entire image. 

In the newer versions of the software, the ROI should be saved with the
image. Otherwise, retrieve acc to Leoncio's advise. 

Best,
 Holly
__________________
Holly L. Aaron
CRL Molecular Imaging Center
http://imaging.berkeley.edu

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Yi Zheng
Sent: Wednesday, July 23, 2008 11:39 AM
To: [log in to unmask]
Subject: Question about LSM510 software

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear all,

I am practice FRAP on Zeiss LSM 510-META.

Right now I do know how to induce the bleach and record the recovery curve 
of ROI(by using click "MeanROI" in time series control ). But everytime at
the 
end of recording, only the curves are saved. So my question is can I save
the 
series of image data as well as the  curve?

Another question is after induce bleach I need to restart the scanning and 
draw the ROI to measure the mean intensity of them. I always worry about 
that I might miss the ROI where bleaching happens. Does anyone here know 
how to do it in a proper way? 

I really appreciate any help!

Thanks,


Yi