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G'day dries,
 
As an alternative to the coverslips that Beat recommends, you should also be able to order special confocal thickness coverslips from Menzel, by special order in any size you wish. The thicness recommended for confocal, especially if you are going to use a water immersion lens is "Grade: 0.170 +/- 0.01mm". We order larger round coverslips of this thicness through an Australian agency (Lomb), but they custom order them from Menzel.
 
This has been discussed previously on the list, se subject:
"Coverslip - Good News"
 
Cheers
Stephen H. Cody
Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008 Royal Melbourne Hospital
Parkville, Victoria,      3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: [log in to unmask]
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________________________________

From: Confocal Microscopy List on behalf of Beat Ludin
Sent: Fri 22/08/2008 9:04 PM
To: [log in to unmask]
Subject: Re: Round coverslips for immunostaining



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Hi Dries

I would recommend the thickness-selected 0.17mm (=type 1014)
coverslips from "assistent" (http://www.hecht-assistent.de <http://www.hecht-assistent.de/> ). Use 12mm
coverslips for 24-well plates. Larger coverslips are difficult to get
out of the wells.
No commercial interest.

Beat


At 10:53 22-08-2008, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>Dear all,
>
>I'd need round coverslips for immunostaining cells in wells of
>24-well plates. Therefore, I was looking for appropriate coverslips
>at Menzel. But as you can see
><http://www.menzel.de/Cover_Slips.675.0.html?L=1>http://www.menzel.de/Cover_Slips.675.0.html?L=1,
>Menzel has only coverslips of class 1, instead I'd need a 0.17mm
>thickness (which is best approximated in class 1.5).
>Thus, my questions are;
>    * what is the ideal diameter of the round coverslips to place
> them in 24-wells? 12, 13 or 14 mm?
>    * Is there another possibility (another company) to buy high
> quality coverslips of #1.5?
>Many thanks in advance for the advice!
>
>Take care,
>
>Dries.
>--
>Dries Vercauteren, PhD student
>Master of Bioscience Engineering: Cell and Gene Biotechnology
>
>Ghent Research Group on Nanomedicines
><http://www.ugent.be/fw/en/research/biofys>www.ugent.be/fw/en/research/biofys
>Faculty of pharmaceutical sciences, Ghent University
>Harelbekestraat 72, 9000 Ghent
>Belgium
>
>Phone: +329/264 80 49
>Mobile: +32485/30 69 80
>E-mail: [log in to unmask]
><mailto:[log in to unmask]>[log in to unmask]