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August 2008

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Subject:
Re: poly-L-lysine : internal reflection
From:
CONFOCAL TL Andresen <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 27 Aug 2008 17:42:52 +0200
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Hi Chris

Yes, when you stain with FITC it will also react with poly-lysine. And
you will naturally get huge background fluorescence. You need to use a
fluorofor that does not contain a isothiocyante group (which mean TRITC
is no good either).

Best regards, Thomas

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Chris Katnik
Sent: Wednesday, August 27, 2008 5:12 PM
To: [log in to unmask]
Subject: poly-L-lysine : internal reflection

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Is there ever a problem with using ploy-L-lysine coated coverslips when
doing 
confocal imaging of FITC or Alexa Fluor 555 stained cells and internal
reflection 
of the laser light?

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