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Farid,
I think that the processing of the tissue is correct.
I wonder that how the xenografts were produced.
DAPI signal appeared as punctate foci within nuclei and accumulated on the 
edges of nuclei
Is the sign of apoptosis or necrosis
Did the investigator inject the cells into the animal with metrigel or 
other reagents to keep the cells alive?
If not, ischemic necrosis will happen in the center of tumor.
The another problem was that how the tumor was harvested. Did they fixed 
the tissue right away?
Those problems can solved by checking the histology of HE stained section.
Mei Ling


At 08:43 PM 8/27/08, you wrote:
>Search the CONFOCAL archive at 
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>Howdy Group,
>Thanks so much to all for excellent suggestions and comments. I will work 
>with my colleague to sort through the comments and our procedures to 
>figure out what we can do better or different.
>
>Best to all.
>Farid
>
>
>--
>Farid Jalali MSc
>Senior Research Technician- Lab Manager
>Applied Molecular Oncology/ Princess Margaret Hospital
>STTARR Innovation Facility/ Radiation Medicine Program
>Toronto, Canada
>416-946-4501 X4351 (Princess Margaret Hospital)
>416-581-7754 STTARR at MaRS Building
>416-581-7791 STTARR Microscopy Suite