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Hello,

Here's question that I've been asked that doesn't seem possible to do, but I 
wonder if anyone has already done it? One of our users has a fixed sample 
with 3 fluorophores (for ease of explanation blue, green and red) and would 
like to image all three channels, but with different z setting depending on 
the channel. She would like a single medial optical section of the blue and 
red channels, but a full z-series from the green channel.

We have both Zeiss (from the LSM 510 series) and Leica (SPE and SP5) 
confocals available to us.

I know this is easy by doing 2 different scans: 1 2-channel scan of the blue 
and red, then a second scan of a z-series of the green, BUT is it possible 
to do this at the single click of a button to save time and user input. It 
is possible on the SP5 using the live data mode, but this scope is really 
reserved and heavily used for live cell imaging. Our SPE doesn't have live 
data mode and therefore applies any Z settings to all sequential scans 
(generating unnecessary quantities of data and taking too long).

Any suggestions welcome,
Thanks,
Graham