Hi Julia
The FRET data analysis software in the Olympus system is based on our algorithm. I think they did a good job in integrating the algorithm in their system.
As you know we run a annual FRET workshop during March to train the participants on FRET data acquisition and processing using various microscope systems including wide-field, spinning disk confocal, laser scanning confocal, Spectral imaging, 2photon, lifetime imaging, acceptor photobleaching, etc.
You should consider attending the course so that you can train others and the software is free for the participants and it works with imageJ. We also added many functions...it can handle 8-bit, 10-bit, 12-bit, 14-bit, 16-bit images, time lapse and 3D FRET, etc. We can also add functions depending on the workshop participants request.
There is no fee for registration and you can register to keep your spot. You can visit the web site to see the info
http://www.kcci.virginia.edu/workshop/index.php
Hope this helps
ammasi


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of js1719
Sent: Wednesday, October 15, 2008 12:56 PM
To: [log in to unmask]
Subject: FRET on FV500

Hello,
  I have a user that is going to do FLIM-FRET on our Olympus FV500
system soon and I was wondering if any other people on the list serve
have an established (written) protocol for doing either de-
constructive acceptor photo-bleaching (using the protocol processor
method) or synthesized emission. We added the FRET module a few years
ago but no one here ever used it. He is set up to use CFP/YFP. I have
told him to do CFP and YFP alone as controls and I think I will teach
him the de-constructive method first since as I remember it being
easier. I also wondered what analysis program people use for
calculating FRET these days- we tend to use ImageJ a lot for analysis
so if you have a plugin that works well can you let me know? I
appreciate all the advice you can give me, this is user is completely
new to microscopes so the more I can provide him with the better.
Best,
  Julia

Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[log in to unmask]
http//:www.kcci.virginia.edu
************************
Workshop on FRET Microscopy, March 3-7, 2009
http://www.kcci.virginia.edu/workshop/workshop2009/index.php
 *************************