Hi,

For widefield imaging, basically you just have to project an (enlarged)
image of the light guide output at the objective back focal plane (or a
conjugate plane, such as where the aperture diaphragm is located). So all
you need is an achromat lens with a suitable focal length that images your
light guide output on the aperture diaphragm.

Best regards,

Kevin



> -----Oorspronkelijk bericht-----
> Van: Confocal Microscopy List [mailto:[log in to unmask]]
> Namens Thomas Aabo
> Verzonden: donderdag 23 oktober 2008 14:27
> Aan: [log in to unmask]
> Onderwerp: Setting up epifluorescence
> 
> I am building my own epifluorescence setup with a monochromator (400-
> 700 nm) as light source. The light guide from the monochromator has an
> N.A. of 0.22. The objective is an Olympus LMPlan IR 50X, N.A. 0.55.
> What would I need, in terms of correcting optics between my light guide
> and the objective rear aperture? And how should the light be focused on
> the objective rear aperture.
> 
> Thomas
> 
> -------------------------------------------------------------
> Thomas Aabo
> Phone: +45 35333636
> M.Sc., Ph.D student, [log in to unmask]
> University of Copenhagen
> Department of Food Science
> Rolighedsvej 30
> 1958 Frederiksberg C
> Denmark
> www.ifv.life.ku.dk
> -------------------------------------------------------------