Anchal-
The easy way is to homogenize the cells and spin down the microsomes (membrane compartments).  If they have the protein upon SDS-PAGE or western blottinf, then it is there still.
Carol Heckman
Center for Microscopy & Microanalysis
Bowling Green State University
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From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Anchall ............ [[log in to unmask]]
Sent: Thursday, November 13, 2008 9:56 PM
To: [log in to unmask]
Subject: Image analysis

Hello all,
           This query is regarding image analysis.I am lookin for some way I can quantify the following data.
I have images with a protein A usually localised in ER and Golgi.But when I overexpress another protein B which kind of solubilises PROTEIN A then I see diffuse expression of protein A all over the cell basically in cytoplasm.Now what I want to find out is whether the protein is still present in ER & Golgi but it is not so distinguisable as now there is lots in cytoplasm too.Is there any way I can quantify this.. using IMAGE J as i mostly use only that...
I hope some help in this regard..
Thanks
Anchal