CONFOCALMICROSCOPY Archives

February 2009

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Chris Tully <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 13 Feb 2009 11:35:10 -0500
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Michelle,

You may want to check out the color correction and measurement
facilities in Image-Pro Plus (http://www.mediacy.com).  Although I no
longer work for the company, I was involved in the development and
testing of these tools.

I am recommending this because in my experience most people who feel
that they need to work directly with the pixel intensity values really
don't understand digital images and/or what they are trying get out of
the image.  Usually a few minutes working with a specialist in image
processing and analysis can reveal tools and methods that achieve the
desired results with far less effort.  Working directly with pixel
intensity values is neither easy or fast!  This is why both commercial
and free programs for image analysis have been developed.

-Chris

Chris Tully
Microscopy and Image Analysis Expert
[log in to unmask]
240-888-1021
http://www.linkedin.com/in/christully



On Fri, Feb 13, 2009 at 10:31 AM, Michelle Peckham
<[log in to unmask]> wrote:
> A little bit off the topic, but
>
> Someone here was asking me about analysing a 'RAW' file for colour
> information, and the simplest way to do what she wants would be to extract
> the grey scale values out for the red, green and blue channels, and analyse
> them in a spread sheet.
>
> Does anyone know how to do this?
>
> Michelle

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