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Date: | Tue, 10 Feb 2009 11:50:58 -0600 |
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Hello,
We recently replaced the power supply (all five subunits) for our
Bio-Rad 1024 control tower. After starting the system, there was an
obvious distortion along the X-dimension in all images. It appears
that the X galvo is zoomed by a factor of ~3.6, resulting in
magnification along only the X-dimension. The characteristic scanning
sound produced with a zoom factor of 1 (no zoom) is not present,
suggesting that indeed the galvo is set to a zoom of ~3.6. An image
of 15-micron green beads collected with the system can be seen here:
http://www.keck.bioimaging.wisc.edu/15umbeads.jpg. All other aspects
of the system work fine, including the zoom function, which allows
zooming but maintains the distortion. The software calibration
settings do not appear to have changed.
Any insights into the cause of the problem (and how to fix it) would
be greatly appreciated.
Thanks,
Michael Hendrickson
Assistant Manager
W.M. Keck Laboratory for Biological Imaging
University of Wisconsin-Madison
www.keck.bioimaging.wisc.edu
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