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March 2009

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Subject:
Autofluorescence on cells using FITC filter
From:
"B. Prabhakar Pandian" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 24 Mar 2009 17:29:31 -0500
Content-Type:
text/plain
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Hello,
             We are having some issues with imaging of cells using FITC 
filter for GFP expression. We are seeing that fraction of cells are 
showing fluorescence when viewed via the FITC filter with no
transfection reagent present.  We have tried playing with exposure 
times, different samples, glass, plastic, etc. Can anybody advise.

Thanks,

-Prabhakar

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