CONFOCALMICROSCOPY Archives

March 2009

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
GFP/RFP quenching
From:
Scott Howell <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 4 Mar 2009 07:52:01 -0500
Content-Type:
text/plain
Parts/Attachments:
text/plain (17 lines) 
List,

We have had some issues here with a lab where the GFP/RFP brightness in
fixed cells has become extremely variable. Like night and day. Wondering
if it may be related to the pH of their paraformaldehyde fix? Does one
particular pH seem to work best?? Any other ideas? Thanks. 

Scott J. Howell, Ph.D.
Manager, Imaging Module
Visual Sciences Research Center
Case Western Reserve University
2085 Adelbert Rd. 
Institute of Pathology Room 106
Cleveland, Ohio 44106
216-368-2300
http://www.case.edu/med/vsrc/

ATOM RSS1 RSS2