Hi Again List,
Yesterday we tried some KY and some ultrasound gel (non-blue version) with great success. Mangaged to image lymphatic vessels in the mouse tail without any real problems (other than geting the mouse where wewanted it). The KY was a bit to runny and didn't stay on the sample for very long. The ultrasound gel worked really well, though the image was a bit fuzzy. I am thinking this is probably due to bubbles in the gel. For those that have used ultrasound gel have you tried degassing it to get rid of small bubbles?
Cheers
Cam
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: Tuesday, 24 March 2009 11:02 PM
To: [log in to unmask]
Subject: Re: Optical Coupling Gels
Actually I've never used Stephen's condom technique, but we use KY jelly all the time in plant physiology. You can seal a chamber to a leaf with it to measure gas exchange, for example, and just rinse it off afterwards. But a bottle lasts a long time (much longer than one used as intended!) so there's not much embarrassment factor involved. I'd never thought to check its optical properties. As I said before, since it's intended for use in body cavities I'd have thought it should be close to isotonic, but I don't know.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of simon walker (BI)
Sent: Tuesday, 24 March 2009 10:37 PM
To: [log in to unmask]
Subject: Re: Optical Coupling Gels
Is this to combine with Stephen Cody's "special" technique? It could raise some eyebrows with lab shelves stocked with these items..
Simon
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: 24 March 2009 09:48
To: [log in to unmask]
Subject: Re: Optical Coupling Gels
KY jelly is used mostly as a sexual rather than surgical lubricant, but its function is the same in both cases.
I'm sure an equivalent product can be found in any pharmacy. It'll be next to the condoms.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Steffen Dietzel
Sent: Tuesday, 24 March 2009 8:39 PM
To: [log in to unmask]
Subject: Re: Optical Coupling Gels
At 02:50 24.03.2009, you wrote:
>Hi All,
>
>Thanks for all the responses. Looks like it is time to lube up the
>objective and......
>
>I have got hold of some Ultrasound gel from the hospital here. The
>stuff i have is a pale blue colour. Is this like the stuff you have
>Steffen?
Nope. Our hospital has two kinds, the "normal"
one to which my previous post applies is colorless. They also have another one, extra-viscous, which is blue. But when I saw on the label of the blue one that it contains 80% Glycerol, I didn't even try that. No idea whether this is some kind of international color code or whether it changes from one block to the next.
Alternatively, you may be able to mix it
yourself: Order 1,2 Propylenglycol and extract the superabsorber from a disposable diaper (unused)....
BTW, apparently KY-Jelly is a brand better known in some countries than in others, anyway I had to look it up. The respective Wikipedia article says "K-Y Jelly's original stated purpose was as a <http://en.wikipedia.org/wiki/Surgical_lubricant>surgical
lubricant, and it was often chosen by doctors because of its natural base. " I didn't even know that surgical lubricants exist, but if such a thing is still in use today, it might also be an alternative for immersion. 'Ask your local doctor or pharmacist if you have any questions or concerns about using this medicine...'
Happy lubricating
Steffen
>Cheers
>
>
>Cam
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