Claire,

You didn't mention how the user is culturing the monolayer, but if 
suspended membranes (milliwell, transwell, etc) are being used, why 
not put the dye into the basal media?

Andy

At 08:13 AM 3/11/2009, you wrote:
>I have a user who is working with polarized epithelial cells and 
>wants to label the basal-lateral membrane with dye. She tried FM4-64 
>but it is not penetrating the tight junctions so it is only labeling 
>the apical surface. She wants to do this on live cells and would 
>prefer a dye to a fluorescent protein. Any suggestions would be helpful.
>
>Sincerely,
>
>Claire
>
>Claire M. Brown, PhD
>Life Sciences Complex Imaging Facility Director
>McGill University Department of Biochemistry
>Montreal, Quebec, H3G 0B1
><http://www.lifesciencescomplex.mcgill.ca/imaging>http://www.lifesciencescomplex.mcgill.ca/imaging
>

Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)