The Leica SP2 was not OS9 - it was Win 2000. The older Leica systems were OS9 - a very good OS in its time but there's no denying Leica did persevere with it beyond its use-by date. Nor was it a pico-second laser (that was not very good) we had a femtosecond Coherent Mira. (The Cornell patent was never taken out in Australia).
The funny thing was that in spite of pioneering ND detectors on the 1024, when Bio-Rad brought out their Radiance MP (which was about the time we were making our purchase decision) it did NOT have ND detectors! They introduced them later- and I remember very well one SPIE conference whan they finally had a Radiance MP with ND detectors. They had a lunchtime demo and would not show them - so after the crowd had left I sat down at the system and tried them. Instantly hugely better images than they's been getting in the demo. And they had one potential customer (not me - it was too late for me) who had been desperate to see these detectors in action! You've got it - they had a great product and a keen customer but would not bring them together. No wonder they went under. It was very sad for me - I'd known the people involved from 1987, and had brought two Bio-Rad confocals. But one cannot run a core facility on sentiment.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net <http://www.guycox.net/>
________________________________
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Eric Scarfone
Sent: Thursday, 14 May 2009 10:51 PM
To: [log in to unmask]
Subject: Re: Recommendations for commercial multi-photon system purchase
Thanks for the precision Guy. You must be at least as old as I am!
2000 was about the time I installed the Biorad 1024 MP back in France. Did your Leica system actually worked back then (I mean with pico second laser, OS 9 and all?) ;=)
Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet
Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden
Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876
email: [log in to unmask]
http://www.ki.se/cfh/
----- Original Message -----
From: Guy Cox <[log in to unmask]>
Date: Thursday, May 14, 2009 3:15 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [log in to unmask]
> Just to keep the record straight, our Leica MP system has non-
> descanneddetectors in both transmission and epi directions - this
> was delivered
> in 2000, ie 9 years ago. We were a fairly early customer for
> these. It
> was (IMHO) a better implementation than the Bio-Rad one (less
> sensitiveto room light). Zeiss back then did have non-descanned
> detection but
> only in transmitted direction - it worked quite well so long as your
> sample wasn't impossibly thick.
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861 http://www.guycox.net
> ______________________________________________
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]On Behalf Of Eric Scarfone
> Sent: Thursday, 14 May 2009 12:46 AM
> To: [log in to unmask]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
> Hi Lisa
> Lucky you to purchase such a machine!!
>
> The beauty with multiphoton excitation is that optical sectioning
> is
> achieved by the limited volume within which photon density is
> sufficient
> for 2 (or more) quasi simultaneous photon absorption events to
> take
> place. For a given laser setting, the thickness within which this
> occurs
> is only determined by the NA of your objective. ALL the
> fluorescence
> coming from your sample is emitted by this NA determined plane.
> There is
>
> no "out of focus" fluorescence. This is very different from Confocal.
>
> To make full use of this one would like to collect as much of the
> fluorescent photons emitted by the samples as possible. The most
> effective way to do that is to use non-descanned external
> detectors both
>
> in the reflected pathway and in the transmitted pathway.
>
> To my knowledge this was implemented first by Warren Zipfel in
> Watt Webb
>
> lab at Cornell and then commercialized by Bio-Rad on the last
> system
> they put on the market before diseappearing at the end of the
> previous
> century.... After a pretty long absorption/digestion process this
> was
> implemented rather recently by Zeiss.
> Others might have come up with similar solution but I do not know
> about
> it.
>
> I'd were you, for this level of price, I'd ask for an on site test
> with
> your own samples. Do not rely on "in factory" demos!!
>
> Cheers
> Eric
>
>
> Eric Scarfone, PhD, CNRS,
> Center for Hearing and communication Research
> Department of Clinical Neuroscience
> Karolinska Institutet
>
> Postal Address:
> CFH, M1:02
> Karolinska Hospital,
> SE-171 76 Stockholm, Sweden
>
> Work: +46 (0)8-517 79343,
> Cell: +46 (0)70 888 2352
> Fax: +46 (0)8-301876
>
> email: [log in to unmask]
> http://www.ki.se/cfh/
>
>
> ----- Original Message -----
> From: "Cameron, Lisa" <[log in to unmask]>
> Date: Wednesday, May 13, 2009 4:14 pm
> Subject: Recommendations for commercial multi-photon system purchase
> To: [log in to unmask]
>
> > I have been investigating commercial multi-photon systems for
> > awhile in order to
> > purchase a system for my Institute's core microscopy facility.
> Our
> > interest is
> > to have the capability to do intravital imaging on an upright
> > stand, but also be
> > able to have facility users be able to put slides on and use the
> > visible scanner
> > and detectors. I realize this is a tall order for such
> versatility
> > in one
> > system, but since it is for a core (which needs to bring in
> > revenue), I'm
> > looking for the most flexible system. Does anyone have any
> > suggestions about the
> > most recent systems on the market? Or could you point out
> factors
> > you think are
> > the most important for making the decision on which company to
> go
> > with? Please
> > feel free to contact me off line.
> >
> > I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus
> MPE
> > and Prairie's
> > system.
> >
> > (BTW - my own experience is with widefield and confocal live-
> cell
> > imaging, so I
> > have not done 2-p myself, but have been learning about it for
> about
> > a year)
> >
> > Thanks!
> > - Lisa
> >
> > ---------------------------------------
> > Lisa Cameron, Ph.D.
> > Director of Confocal and Light Microscopy
> > Dana Farber Cancer Institute
> > 44 Binney St.; JF 215
> > Boston, MA 02115
> > Office phone: 617-582-8824
> > Fax: 617-582-8750
> > [log in to unmask]
> >
> >
> >
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